Curriculum Vitae

Dr. Hoda  Ayat

 PhD in Molecular Genetic

Personal Information
	Born	February 19th, 1976 in  Isfahan, IRAN
	Present address	P.O. Box: 115- Department of Genetics – School of science- Shahrekord University-Shahrekord- Iran
	Mobile /Telephone	03814424406
	E-mail	ayat-h@sci.sku.ac.ir
	Alternative Email	hoda.ayat@gmail.com

Occupation
	Assistant Professor In Molecular Genetic in Shahrekord University.

Education
	2006	Ph. D. in Molecular Genetics, University of Tarbiat odarress, and National Research Center of Genetic  Engineering and Biotechnology (NRCGEB). Thesis: Generation and characterization of human recombinant  monoclonal antibodies specific for Her-2 and CEA tumor markers using phage display technology. Supervisor: Dr Mehdi Arbabi
	2001	M.Sc. in Genetics, Department of Genetics, Faculty of Science, University of Tarbiat Modarress, Tehran,Iran.   Thesis: Detection of Varicella Zoster Virus Gene 52 Expression by                                DNA Vaccine Technique.   Advisor: Dr. Majid Sadeghizadeh
	1997	B.Sc. in Molecular Biology, Faculty of Science, University of  Isfahan, Isfahan,Iran.

Languages
	Persian English

Awards
	1997	Isfahan University Award for top undergraduate students

Honors
	1999	Top student in B.Sc. course in Molecular Biology.
	1999	Number two candidate in genetic in national M.Sc. exam
	2001	Number two candidates in Molecular genetics PhD exam.

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Teaching Experience
2005-present	Molecular Genetics, Genetic Engineering, Immunogenetics, Cytogenetic. B.Sc. and MSc. Students. Molecular Methods (PhD students) Shahrekord University.

	Technical Experience
		1.                      Molecular cloning methods 2.                      Phage Display 3.                      Mammalian cells culture 4.                      FACS: Fluorescent Activating Cell Sorting
	Membership and collaboration in scientific Associations
	1.      Iran Biology Associations 2.      Shahrekord university Biotechnology committee

Research Experience

·         Accomplished and Ongoing Researches :
	1.      Phage display. 2.      Isolation and preparation of cDNA library of Iranian Scorpion for medical and phylogenic studies. 3.      Cloning and expression of genes encoding biological toxins(scorpion and other invertebrates in prokaryotic systems. 4.      Cloning and expression of fusion proteins in prokaryotic system. 5.      Construction of camel antibody library against Human Tumor Antigens. 6.      Protein engineering design of Diabodies. 7.      Recombinant viral Vaccines.

As Supervisor in MS.c. Thesis:
1	Elham Behvandi. Construction of CDNA Library from camel pool antibodies against human tumor markers. Shahrekord University, Graduated.
2	Sheyda Ilkhanizadeh, Sequencing and cloning of Iranian scorpion “Mesobuthus” cholorotoxin in prokaryotic system. Shahrekord University, Graduated.
3	Elham Asadi, Comparative study of IGF-1 and OCT4 genes expression in early stages of embryo development by using of Semi Quantitative-RT-PCR method, Shahrekord University, Graduated.
4	Nayersadat Fatemi, Effect of Oxidative stress on MicroRNAs expression in testis of mature mouse. Shahrekord University,

As Advisor in MS.c. and Vetinary Science Thesis:
1	Mohamad reza Hajjari. Designing and counstructing of the gene sequence 0f metal binding protein by PCR with overlapping oligonucleotides and its cloning in E.Coli. Shahrekord University, Graduated.
2	Atefeh Sharifi-rad, Cloning and expression of functional domains of timp-4 gene in prokaryotic system.Shahrekord University. Graduated.
3	Amin Izaditabar. The effect of Gabapentin and Clonazepam treatment in induction of mdr1a gene expression in animal model. Shahrekord University,
4	Somayeh khatami. sequencing and study of promoter region of ETF3 gene in vertebrates and its association with early abortion and expression analyses of it in embryonic cells cocultured with Mesenshimal stem cells. Shahrekord University,
5	Maryam Hemmat zadeh. Identification and expression of toll like receptors(TLRs) in sheep reproductive system. Shahrekord University.

Publications
	1.      Sadeghizadeh M., Ayat H, Mirshahi M.; Detection of Varicella Zoster        Virus Gene 52 Expression by DNA Vaccine Technique, J. of Modarres       Medical Science, 2003; 6(2):85-93.
	2.      Ayat  H., Rastgo N., Jahanzad E., Sadghizadeh M., Arbabi  M.;     Construction of Human Recombinant ScFv Phage Llibraries from the  Advanced Stages of  Breast Carcinoma Patients. Iranian Journal of Biotechnology, 2005; 3(2): 170-179
	3.      Ali Mohammad Ahadi , Farnaz Farzaneh Dehkordi, Abolfazl Shirazi, Hasan Nazari, Ebrahim Ahmadi, Hoda Ayat, Majid Sadeghizadeh. Gender determination in single bovine blastomeres by multiplex nested single cell polymerase chain reaction(MNSC-PCR). J. Iran. Chem. Soc., Vol. 6, Suppl., November 2009, pp. S103-S114
	4.   Khademi S., Ahadi AM., Mehvari J., Ayat H., Farrokhi E., Moradi MT., Hashemzadeh Chaleshtori M., Detection of A1430G mutation in SCN1A gene in a patient affected by GEFS-Like Epilepsy in Chaharmahal and Bakhtiari Province. J of  Shahrekord Medical Science University. Accepted.2011.
	5.      Ilkhani zade S, Ayat H., Ahadi AM., Pirali K., Sequencing and Comparative-Bioinformatic Analysis of Chlorotoxin-Like Peptide from the Iranian Scorpion Mesobuthus eupeus. J of  Shahrekord Medical Science University. Accepted.2011.
	6.   Ayat H., Arbabi M., Borruno O., Sadeghzadeh M.,; Isolation of Tumor Specific Internalizing Human scFv Antibodies by Selection of an antibody phage library using tumor cells. In proceeding.
Conferences
		2001	Sadeghizadeh M., Ayat H, Mirshahi M.; Study of Varicella Zoster Virus Gene 52 Expression by DNA Vaccine TechniqueIn vivo and In vitro. 2nd International Congress of Biotechnology, Karaj, Iran. October
		2006	Ayat H., Arbabi M., Sadeghzadeh M., Jahanzad E.; Isolation of Tumor- Specific Internalizing Human scFv Antibodies by Selection of an antibody phage library using tumor cells. 14th National & 2nd International Conference Biology, Tehran, Iran
		2009	Solmaz Khademi, Ali Mohammad Ahadi, Morteza Hashemzadeh Chaleshtori,Jafar Mehvari, Hoda Ayat, Effat Farrokhi. Study of etiological role of ion channels in patients affected by Idiopathic Generalized Epilepsy (IGE) in Chahar Mahal va Bakhtiyari Province.first National conference of biology Student. Shahrekord university. Shahrekord.
		2009	Reihaneh Farghadani, Solmaz Khademi, Ali Mohammad Ahadi, Hoda Ayat, Mehran Arabi. The homology study of some Hox family genes between invertebrates and human. first National conference of biology Student. Shahrekord university. Shahrekord.
		2009	Sharifirad A., Ahadi A M., Ayat H., Shirazi A., Ahmadi E., Nazari H. Analysis of homology between otx2 sequences of Iranian sheep and other close related species.first National conference of biology Student. Shahrekord university. Shahrekord.
		2009	Ahadi A M., Ayat H., Self citation and Plagiarism.first National conference of scientific development and revolution year. Shahrekord university. Shahrekord.
		2009	Ilkhanizadeh Sh., Ayat H., Ahadi A M., Sharifirad A., Pirali K., Analysis of probable prediction of Arachnoid toxins motives contributing to interaction with ion channels. first National conference of biology Student. Shahrekord university. Shahrekord.
		2010	Ahadi A.M., Ayat H., Sadeghizadeh M., Gharagoozli K., Houshman M., Solmaz Khademi,  Study of Parental consanguinity and other diagnostic factors in Iranian patients affected with idiopathic generalized epilepsies. 11th Iran congress on Genetics. Shahid Beheshti University. Tehran, Iran.
		2010	Solmaz Khademi, Ali Mohammad Ahadi, Morteza Hashemzadeh Chaleshtori,Jafar Mehvari, Hoda Ayat. A Missence Mutation in α1 subunit of Sodium-Channel Gene (scn1a) in a patient affected by Severe Myoclonic Epilepsy of Infancy. 11th Iran congress on Genetics. Shahid Beheshti University. Tehran,Iran.
		2010	Ali Mohammad Ahadi, Farnaz Farzaneh Dehkordi, Abolfazl Shirazi, Hoda Ayat, Hasan Nazari, Ebrahim Ahmadi, , Majid Sadeghizadeh. Optimization of diagnostic sensitivity of DNA extension in the standard condition of Multiplex PCR and comparison with Nested Multiplex PCR in sex determination of Bovine. 11th Iran congress on Genetics. Shahid Beheshti University. Tehran, Iran
		2010	Momeneh Foroutan, Ramyar Molania, Atefeh Sharifi rad, Dr .Ali Mohamad Ahadi, Dr .Hoda Ayat. A comparative study between amino acid containing and codon usage in otx and oct gene families. 16th National and 4th International Conference of Biology. Ferdowsi University of Mashhad, Mashhad, Iran.
		2010	Sheyda Ilkhani Zadeh, Dr .Hoda Ayat, Dr .Ali Mohammad Ahadi, Dr.Khodadad Pirali. Sequencing and cloning of Iranian scorpion “Mesobuthus” cholorotoxin in prokaryotic system.16th National and 4th International Conference of Biology. Ferdowsi University of Mashhad, Mashhad, Iran
		2010	Atefeh Sharifi-rad, Dr. Ali Mohammad Ahadi, Dr. Hoda Ayat. Cloning and expression of functional domains of timp-4 gene in prokaryotic system. 16th National and 4th International Conference of Biology. Ferdowsi University of Mashhad, Mashhad, Iran.