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Saturday 8 February 2025




Behnaz Saffar

Associate Professor

Genetics

Basic Science Faculty

Tel: 03832324401

saffar_b@sci.sku.ac.ir

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Research Interest
   Genetic engineering:
   Production of recombinant protein:
   Gene cloning:
   virus gene silensing:
   DNA Vaccine:

Academic Resume
   Phd, , , Iran، Graduation Date: 2006
   Master, , , Iran، Graduation Date: 1998
   Bachelor, , Shahid Beheshti University, Iran، Graduation Date: 1996

Publications
International Journal
1. Shams, Mokhtari, Kazemi, Saffar, Cloning of BHV-UL۲۵ Conserved Fragment in a Lentiviral Transfer Plasmid for the Preparation of a Monitoring Cell Line ,
2. M khorami , A Mokhtari, B Saffar, Cloning of a BDV-NS۳ Conserved Fragment for the Preparation of a Reporter Cell Line ,
3. B Saffar, M Alijani et al, Immunological evaluation of a novel multi-antigenic DNA vaccine encoding SAG۱, SAG۳, MIC۴, GRA۵, GRA۷, AMA۱and BAG۱ against Toxoplasma gondii in BALB/c mice ,
4. لیلا اسدی بهناز صفار اعظم مختاری, In silico design of short hairpin RNA (shRNA)Molecules for DNA pol gene of Contagious Ecthyma virus (ORFV) ,
5. مرجان عباسی روح اله همتی بهناز صفار, Thermally stable and acidic pH tolerant mutant phytases with high catalytic efficiency from Yersinia intermedia for potential application in feed industries ,
6. اعظم مختاری الهه امجدی بهناز صفار, RNA interference targeting UL۲۵ gene as a gene therapy approach against BHV-۱ ,
7. سمیه ریسی بهاره رفیع زاده بهناز صفار, Anti-cancer Effect of Recombinant PI-Laterosporulin۱۰ as a Novel Bacteriocin with Selective Cytotoxicity on Triple Negative Breast Cancer Cells ,
8. محمد جواد حاجی حسنی، اعظم مختاری بهنازصفارلیلا اسدی, In vitro Inhibition of Border Disease Virus Replication With Lentivirus-Mediated shRNAs ,
9. ثریا مظفری، ، زهرا ایزدی، روح اله همتی ، بهناز صفار, Fabrication of an ultrasensitive aptasensor for precise electrochemical detection of the trace amounts of streptomycin in ,
10. , The Construction of a Short Gene by a Very Fast, Modifiedand Simplified Gene Synthesis and the Analysis of Various ,
11. , Translational selection on SHH genes ,
12. , Amplification, Sequencing and Cloning of Iranian Native Bacillus subtilis Alpha-amylase Gene in Saccharomyces cerevisiae ,
13. , Design of a novel metal binding peptide by molecular dynamicssimulation to sequester Cu and Zn ions ,
14. , Improvement of Cd۲+ uptake ability of SmtA protein by Lys/Cys mutation; experimental and theoretical studies ,
15. , Cloning, Codon Optimization and Expression of Yersinia Intermedia Phytase Gene in E.coli ,
16. , Functional Analysis of Recombinant Codon-Optimized Bovine Neutrophil ?-Defensin ,
17. Lila Asadi, Behnaz Saffar, Azam Mokhtari, Ehsan Arefian, Lentivirus expressing shRNAs inhibit the replication of contagious ecthyma virus bytargeting DNA polymerase gene ,
18. , Multi-function Plant Defensin, Antimicrobial and Heavy Metal Adsorbent Peptide ,
19. , Experimental and theoretical studies of cadmium ions absorption by a new reduced recombinant defensin ,
20. , Evaluation of smtA expression and E. coli survival against cadmium ions ,
21. , Cloning of a Conserved Domain of ORFV- DNA polymerase in the pCDH-CMV-MCS-EF۱- cGFP-T۲A-Puro Lentiviral Plasmid ,
22. , Isolation of Taxol-Producing Endophytic Fungi from Iranian Yew Through Novel Molecular Approach and Their Effects on Human Breast Cancer Cell Line ,
23. , Functional expression and purification of recombinant Hepcidin۲۵ production in Escherichia coli using SUMO fusion technology ,
24. , Super RLuc۸: A novel engineered Renilla luciferase with a red-shiftedspectrum and stable light emission ,
Research Journal
٢٥. نسرین مسعودی مجسن مبینی بهناز صفار, بهينه سازي توليد آلفا آميلاز با استفاده از باکتري باسيلوس سابتيليس در شرايط تخمير بستر جامد و خالصسازي جزئي آنزيم PTCC ۱۷۲۰ ,
٢٦. زهرا توکلی بهناز صفار کریم مهنام, طراحي،کلونينگ، بيان و خالص سازي پپتيد هيستاتين ۳ جهش يافته و بررسي اثرات ضد ميکروبي آن ,
٢٧. معصومه یوسف زاده روح اله همتی بهناز صفار, جداسازي و شناسايي باکتري سرمادوست از روده سخت پوست گاماروس جدا شده از چشمه هاي آب سرد شهر سامان در استان چهارمحال و بختياري وارزيابي آنزيم هاي مختلف آن ,
٢٨. نسربن مسعودی محسن مبینی بهناز صفار, بهينه سازي توليد آلفا آميلاز با استفاده از باکتري باسيلوس سابتيليس PTCC ۱۷۲۰ در شرايط تخمير بستر جامد و خالصسازي جزئي آنزيم ,
29. , Thermal inactivation and aggregation of lysozyme in the presence of nanoTio۲ and Nano Sio۲ in neutral pH ,
30. , Development a New Human Skin Continuous Cell Line Sensitive to Mumps Virus to Mumps Virus ,
31. , Development a New Human Skin Continuous Cell Line Sensitive to Mumps Virus to Mumps Virus ,
٣٢. , Desing,cloning expression and purification of hepcidin 3 peptide and investigation of antimicrobial effects. ,
International Conference
33. اعظم امینی بهناز صفار، اعظم مختاری رلیلا اسدی, Designing shRNA Targeting ۲۰۱۹-novel Coronavirus RdRPs Gene as a Potential Gene Therapy Tool Using Computational Pattern ,
٣٤. محمد گلچین فرزانه محدی بهناز صفار, شناسايي lncRNA هاي مرتبط با ژن هاي در گير در پاسخ به درمان با azacitidine در بيماران ,



Books



Honors



Thesis
Number Student Name Title Defence Date
1. Bahare Nikozar Subcloning and expression of recombinant HLA-A*31:01 for experimental investigation of its interaction with neuroantigenic یکشنبه ٢٠ بهمن ١٣٩٨
2. Margan Manochri Prokaryotic expression and purification of conserved matrix protein I (M1) of influenza A virus, to be used in broad-spectrum یکشنبه ٢٤ شهريور ١٣٩٨
3. Mansoreh Ghorbani Site-directed mutagenesis of T312R and F260R on the active site of phytase from Yersinia intermedia to investigate kinetic properties چهارشنبه ٢٧ شهريور ١٣٩٨
4. Mehri Hedayati Design and molecular dynamic simulation, cloning and expression of recombinant peptide cap37 in E.coli چهارشنبه ٣٠ بهمن ١٣٩٨
5. Farhad Banimehdi Design, synthesis, cloning and expression of the anti-cancer peptide LS10 in E.coli یکشنبه ٣١ شهريور ١٣٩٨






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